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- RNA-BEE
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*Faster 1 hour protocol
*Blue dye added to reagent to facilitate organic/aqueous phase separation
*Applications are the same as those of RNAzol
*2 ml isolates RNA from 100 mg tissue or 1 x 107 cells
or 10 x 107cells
- RNA STAT - 60
- *Isolates total RNA and enriches for mRNA in <60 minutes.
*Extracts 30-150% more total RNA/mRNA than any other
method.
*No additional purification is required for Northern blotting, molecular
cloning, RNase protection or PCR.
*1 ml isolates RNA from 100 mg tissue or 10 x 106 cells.
- RNA Stat 50 LS
- *Extraction reagent for liquid samples
*Effective method of RNA isolation from biological fluids (blood,
plasma, amniotic fluid and CSF).
*Excellent for polymerase chain reaction without the hassle of
additional DNase purification.
- DNA Stat-60
- *Isolates high quality genomic DNA in one hour from tissues and cells with
a single monophasic reagent
*Can be used to back extract DNA from "Single Step Method " RNA
Preps (i.e, RNAzol, RNA Bee, RNA Stat-60)
*Does not contain phenol
*RNA and proteins sequestered in organic and interphase; DNA remains in
upper aqueous phase.
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- RNA-DNA Stat
- *Isolates high quality RNA/DNA from same sample in 90 minutes.
*1ml isolates RNA/DNA from 100 mg tissue or 10 x 106 cells
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Aquanase - Free Water
- *Ultra pure RNase, DNase, free water for laboratory use only
*DEPC treated, sterile, non-pyrogenic
*No enzyme inhibition
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High Efficiency Transfer
Solution
- *A 15X concentrated solution of inorganic and
organic salts.
- *High efficiency transfer solution of RNA from agarose gel to
hybridization membranes.
- *Allows visualization of RNA on the membranes
without staining with ethidium bromide.
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- Northern / Southern Transfer Solution
- *A 25X concentrated solution for use in Northern and Southern
blotting without the use of the ethidium bromide.
- *Increases
both the transfer efficiency and intensity of the specific hybridization
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Background Quencher
- * A blocking reagent supplied as a 10x
concentrate designed for use in hybridization.
- * Prevents Non-Specific binding of Hybridization probes.
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High Efficiency
Hybridization Reagent
- * Ready to use hybridization reagent for
Northern, Southern, and dot-blot hybridization
- * Cs-108A and B system is supplemented with 0.9 m NaCl and 1% SDS
- * Cs-108F and G system is supplemented with
0.7 m NaCl and 1% SDS, and 50% formamide
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Low Background
Hybridization Reagent
- * Ready to use hybridization reagent for
Northern, Southern, and dot-blot hybridization
- * Cs-109A and B system is for use at 68°C
- * Cs-109F and G system is for use at 42°C
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- Formazol
- * Inactivates RNases
* High sample volume can be applied to
formaldehyde gels
* Allows - 20°C storage of RNA for at least
one year
* Contains a specially purified and
stabilized formamide
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