Recent progress in RNA isolation technology has made it possible to replace lengthy and laborious methods of total RNA isolation (1) by a single-step method (2,3). The RNA STAT-50TM LS is a new and substantially improved version of the single-step method. It is a complete and ready to use reagent for isolation of total RNA from liquid samples of human, animal, plant, yeast and bacterial origin. Extensive laboratory tests have shown that RNA STAT-50 TM LS is highly reliable and produces very consistent results.
The composition of RNA STAT-50TM LS includes phenol and Guanidinium thiocyanate in a mono-phase solution. A biological specimen is lysed (or homogenized) in RNA STAT-50TM LS and mixed with chloroform. Following centrifugation, the lysate separates into two phases: aqueous phase and organic phase. The total RNA remains exclusively in the aqueous phase while DNA and proteins are extracted into an organic phase and interphase. The total RNA is precipitated from the aqueous phase by addition of isopropanol, washed with ethanol and solubilized in water. RNA STAT-50 TM LS is the most effective method of RNA isolation from biological fluids such as blood, serum, amniotic fluid, CSF etc. The entire procedure can be completed in 1 hour and the recovery of undegraded mRNAs I s30-150% greater than with any other method of RNA isolation.

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